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POSTDOCTORADOS

Dr. Gustavo Núñez

Dr. Gustavo Núñez

Investigador Postdoctoral RP1

gustavonunez@udec.cl

Doctor en Ciencias con Mención en Manejo de Recursos Renovables Acuáticos. Universidad de Concepción. (2014-2018).
Ingeniero en Biotecnología Marina y Acuicultura. Universidad de Concepción. (2005-2010).
Licenciado en Biotecnología Marina y Acuicultura. Universidad de Concepción. (2005-2009).

 

 

Publicaciones
  1. Gustavo Núñez-Acuña, Cristian Gallardo-Escárate, David M. Fields, Anne Berit Skiftesvik, Howard I. Browman. Silencing of ionotropic receptor 25a decreases chemosensory activitity in the salmon louse Lepeophtheirus salmonis during the infective stage. Gene, 697: 35-39.
  2. Cristian Gallardo‐Escárate, Gabriel Arriagada, Crisleri Carrera, Ana Teresa Gonçalves, Gustavo Nuñez ‐ Acuña, Diego Valenzuela ‐Miranda, Valentina Valenzuela ‐Muñoz. The race between host and sea lice in the Chilean salmon farming: a genomic approach. Reviews in Aquaculture, accepted. DOI: 10.1111/raq12334.
  3. Ana Teresa Gonçalves, Gustavo Núñez-Acuña, Camille Détrée, Cristian Gallardo-Escárate. Coding/non-coding cross-talk in intestinal epithelium transcriptome gives insights on how fish respond to stocking density. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 29: 14-23.
  4. Gustavo Núñez-Acuña, Cristian Gallardo-Escárate. Characterization of the salmon louse Lepeophtheirus salmonis miRNome: Sex-biased differences related to the coding and noncoding RNA interplay. Marine Genomics, acceptted. DOI: 10.1016/margen.2019.01.005.
  5. Gustavo Núñez-Acuña, Cristian Gallardo-Escárate, David M. Fields, Steven Shema, Anne Berit Skiftesvik, Ignacio Ormazábal, Howard I. Browman. The Atlantic salmon (Salmo salar) antimicrobial peptide cathelicidin-2 is a molecular host recognition cue for the salmon louse (Lepeophtheirus salmonis). Scientific Reports, 8 (13738): 1-11. DOI: 10.1038/s41598-018-31885-6.
  6. Gustavo Núñez-Acuña, Camille Détrée, Cristian Gallardo- Escárate, Ana Teresa Gonçalves. Functional Diets Modulate lncRNA-Coding RNAs and Gene Interactions in the Intestine of Rainbow Trout Oncorhynchus mykiss. Marine Biotechnology, 19 (3): 287-300.
  7. Camille Détrée, Gustavo Núñez-Acuña, Fabián Tapia, Cristian Gallardo-Escárate. Long noncoding RNAs are associated with spatiotemporal gene expression profiles in the marine gastropod Tegula atra. Marine Genomics, 33: 39-45
  8. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Sebastián Boltaña, Gustavo Núñez- Acuña, Diego Valenzuela- Miranda, Ana Teresa Gonçalves, Camille Détrée, E. Tarifeño- Saldivia, Rodolfo Farlora, Steven Roberts, H. M. Putnam. The Caligus rogercresseyi miRNome: Discovery and transcriptome profiling during the sea lice ontogeny. Agri Gene, 4: 8-22.
  9. Gustavo Núñez-Acuña, Sebastian Boltaña & Cristian Gallardo-Escárate. (2016). Pesticides drive stochastic changes in the chemoreception and neurotransmission system of marine ectoparasites. International Journal of Molecular Sciences, 17 (6): 1-14.
  10. Camille Detreé, Gustavo Núñez-Acuña, Steven Roberts & Cristian Gallardo-Escárate. (2016). RNA-seq analysis reveals a complex defense response against saxitoxin in the mussel Mytilus chilensis. PLoS ONE, 11 (10): 1-20.
  11. Gustavo Núñez-Acuña, Jorge Pino Marambio, Tirza Valenzuela, Simon Wadsworth & Cristian Gallardo-Escárate. (2016). Antimicrobial peptides from Salmon salar skin induce frontal filament development and olfactory/cuticle-related genes in the sea louse Caligus rogercresseyi. Aquaculture, Aquaculture, 464 (1): 171-177.
  12. Gustavo Núñez-Acuña, Freddy Vera-Bizama, Sebastián Boltaña, Cristopher Hawes, Jorge Pino Marambio, Simon Wadsworth & Cristian Gallardo-Escárate. (2015). In-feed additives modulate ionotropic receptor genes from the sea louse Caligus rogercresseyi: a comparative analysis in two host salmonid species. Aquaculture, 451: 99-105.
  13. Gustavo Núñez-Acuña, Ana T. Gonçalves, Valentina Valenzuela-Muñoz, Jorge Pino Marambio, Simon Wadsworth & Cristian Gallardo-Escárate. (2015). Transcriptome immunomodulation of in-feed additives in Atlantic salmon Salmo salar infested with sea lice Caligus rogercresseyi. Fish and Shellfish Immunology, 47: 450-460.
  14. Allisson Astuya, Crisleri Carrera, Viviana Ulloa, Ambbar Aballay, Gustavo Nuñez-Acuña, Hélène Hégaret & Cristian Gallardo-Escárate. (2015). Saxitoxin modulates immunological parameters and gene transcription in Mytilus chilensis hemocytes. International Journal of Molecular Science, 2015 (16): 15235-15250.
  15. Rodolfo Farlora, Gustavo Núñez-Acuña & Cristian Gallardo-Escárate. (2015). Prohibitin-2 gene reveals sex-related differences in the salmon louse Caligus rogercresseyi. Gene, 564 (1): 73-80.
  16. Gustavo Núñez-Acuña & Cristian Gallardo-Escárate. Two novel male-associated peroxinectin genes are downregulated by exposure to delousing drugs in Caligus rogercresseyi. Gene, 557 (1): 98-102.
  17. Diego Valenzuela-Miranda, Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Sassan Asgari & Cristian Gallardo-Escárate. (2015). MicroRNA biogenesis pathway from the salmon louse (Caligus rogercresseyi): Emerging role in delousing drug response. Gene, 555 (2): 231-241.
  18. Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz & Cristian Gallardo-Escárate. (2014). Insights into the olfactory system of the ectoparasite Caligus rogercresseyi: molecular characterization and gene transcription analysis of novel ionotropic receptors. Experimental Parasitology, 145: 99-109.
  19. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz & Gustavo Núñez-Acuña. (2014). RNA-Seq Analysis Using De Novo Transcriptome Assembly as a Reference for the Salmon Louse Caligus rogercresseyi. PLoS ONE, 9(4): e92239.
  20. Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz & Cristian Gallardo-Escárate. (2014). High-throughput SNP discovery and transcriptome expression profiles from the salmon louse Caligus rogercresseyi (Copepoda: Caligidae). Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 10: 9-21.
  21. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Jacqueline Chávez-Mardones & Waleska Maldonado-Aguayo. (2014). Transcriptome analysis of the couch potato (CPO) protein reveals an expression pattern associated with early development in the salmon louse Caligus rogercresseyi. Gene, 536: 1-8.
  22. Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña & Cristian Gallardo-Escárate. (2014). Molecular Characterization and Transcription Analysis of P-Glycoprotein Gene from the Salmon Louse Caligus rogercresseyi. Journal of Aquaculture, Reserach & Development, 5: 236, doi: 10.4172/2155-9546.1000236.
  23. Gustavo Núñez-Acuña & Cristian Gallardo-Escárate. (2013). Identification of immune-related SNPs in the transcriptome of Mytilus chilensis through high-throughput sequencing. Fish and Shellfish Immunology, 35: 1899-1905.
  24. Gustavo Núñez-Acuña & Cristian Gallardo-Escárate. (2013). The myostatin gene of Mytilus chilensis evidences a high level of polymorphism and ubiquitous transcript expression. Gene, 536: 207-212.
  25. Gustavo Núñez-Acuña, Andrea Aguilar-Espinoza & Cristian Gallardo-Escárate. (2013). Complete mitochondrial genome of Concholepas concholepas inferred by 454 pyrosequencing and mtDNA gene expression of two wild populations. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 8(1): 17-23.
  26. Waleska Maldonado-Aguayo, Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Jacqueline Chávez-Mardones & Cristian Gallardo-Escárate. Molecular characterization of two kazal-type serine proteinase inhibitor genes in the surf clam Mesodesma donacium exposed to Vibrio anguillarum. Fish and Shellfish Immunology, 34(6): 1448-1454.
  27. Cristian Gallardo-Escárate, Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz. (2013). SNP discovery in the marine gastropod Concholepas concholepas by high-throughput transcriptome sequencing. Conservation Genetics Resources, 5(4): 1053-1054.
  28. Gustavo Núñez-Acuña, Ambbar E. Aballay, Allisson P. Astuya & Cristian Gallardo-Escárate. (2013). Transcriptional responses of Mytilus chilensis exposed in vivo to Saxitoxin (STX). Journal of Molluscan Studies, 79(4): 323-321.
  29. Jacqueline Chávez-Mardones, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Waleska Maldonado-Aguayo & Cristian Gallardo-Escárate. (2013). Concholepas concholepas Ferritin H-like subunit (CcFer): molecular characterization and single nucleotide polymorphism associated to innate immune response. Fish and Shellfish Immunology, 35: 910-917.
  30. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña & P.A. Haye. (2013). SNP discovery and gene annotation in the surf clam Mesodesma donacium. Aquaculture Research, published online, doi:10.1111/are.12273.
  31. Lafarga-De la Cruz, F., Núñez-Acuña, G., Gallardo-Escárate, C. (2012). Hybridization between Haliotis rufescens and H. discus hannai: evaluation of fertilization, larval development, growth and thermal tolerance. Aquaculture Research, 2012: 1-15. DOI: 10.1111/j.1365-2109.2012.03121.x.
  32. Gustavo Núñez-Acuña, Fabian J. Tapia, Pilar A. Haye & Cristian Gallardo-Escárate*. (2012). Gene expression in Mytilus chilensis populations reveals local patterns associated with ocean environmental conditions. Journal of Experimental Marine Biology and Ecology, 420-421: 56-64.
  33. Gustavo Núñez-Acuña, Andrea Aguilar-Espinoza, Jacqueline Chávez-Mardones & Cristian Gallardo-Escárate*. (2012). Ubiquitin-conjugating enzyme E2 gene associated with pathogen response in Concholepas concholepas: SNP identification and transcription expression. Fish and Shellfish Immunology, 33(4): 1065-1068.
  34. Salinas-Clarot, K., Gutiérrez, A., Núñez-Acuña, G., Gallardo-Escárate, C. (2011). Molecular characterization and gene expression of ferritin in red abalone (Haliotis rufescens). Journal of Fish and Shellfish Immunology, 30(1): 430-433.
Proyectos
Congresos
  1. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda. Reverse Vaccinology: Functional Genomics Applied to the Development of Vaccines against Caligus rogercresseyi. Plant & Animal Genome XXVII 2019, USA, San Diego, 12 – 16 January.
  2. Gustavo Núñez-Acuña, Cristian Gallardo-Escárate, David M. Fields, Steven Shema, Anne Berit Skiftesvik, Ignacio Ormazábal, Howard I. Browman. The Atlantic salmon (Salmo salar) antimicrobial peptide cathelicidin-2 is a molecular host recognition cue for the salmon louse (Lepeophtheirus salmonis). 12th International Sea Lice Conference 2018, CHILE, Punta Arenas, 04 – 06 November.
  3. Gustavo Núñez-Acuña, Gabriel Arriagada, Valentina Valenzuela-Muñoz, Constanza Sáez-Vera, Cristian Gallardo-Escárate. Time-to-response toxicity analysis using molecular biomarkers as alternative bioassays for delousing drug sensitivity. 12th International Sea Lice Conference 2018, CHILE, Punta Arenas, 04 – 06 November.
  4. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda. Reverse vaccinology applied to the development of new vaccines against Caligus rogercresseyi. 12th International Sea Lice Conference 2018, CHILE, Punta Arenas, 04 – 06 November.
  5. Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Gabriel Arriagada, Diego Valenzuela-Miranda, Cristian Gallardo-Escárate. Disentangling sea lice responses to antiparasitic drugs: emerging role from non-coding RNAs. 12th International Sea Lice Conference 2018, CHILE, Punta Arenas, 04 – 06 November.
  6. Gustavo Núñez-Acuña, Cristian Gallardo-Escárate, Anne Berit Skiftesvik, David M. Fields, Howard I. Browman. Silencing of ionotropic receptor 25a decreases chemosensory activity of the salmon louse Lepeophtheirus salmonis during the infective stage. 12th International Sea Lice Conference 2018, CHILE, Punta Arenas, 04 – 06 November.
  7. Gustavo Núñez-Acuña, Cristian Gallardo-Escárate. Characterization of the salmon louse Lepeophtheirus salmonis miRNome: sex-biased differences related to the coding and non-coding RNA interplay. 12th International Sea Lice Conference 2018, CHILE, Punta Arenas, 04 – 06 November.
  8. Gustavo Núñez-Acuña, Howard I. Browman, Anne Berit Skiftesvik, David M. Fields & Cristian Gallardo-Escárate. Key cell-receptors and kairomones involved in host-recognition mechanisms in the sea lice Lepeophtheirus salmonis inferred from RNAi experiments, Congreso Internacional de Biotecnología La Habana 2017, CUBA, Varadero, 03 – 06 December.
  9. Gustavo Núñez-Acuña, David M. Fields, Steven Shema, Howard I. Browman, Anne Berit Skiftesvik, Cristian Gallardo-Escárate. Host recognition mechanisms in the sea lice: genomic and functional approaches to identify novel chemoattractans derived from salmon skin, European Aquaculture Society Conference 2017, CROACIA, Dubrovnik, 18 – 20 October.
  10. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda. Functional genomics applied to the development of new vaccines against the sea louse Caligus rogercresseyi, European Aquaculture Society Conference 2017, CROACIA, Dubrovnik, 18 – 20 October.
  11. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Sebastián Boltaña, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda, Ana T. Gonçalves, Camille Détrée, Estefanía Tarifeño-Silva, Rodolfo Farlora, Steven Roberts, H.M. Putnam. The Caligus rogercresseyi miRnome: discovery and transcriptome profiling during the sea lice ontogeny, European Aquaculture Society Conference 2017, CROACIA, Dubrovnik, 18 – 20 October.
  12. Ana Teresa Gonçalves, Gustavo Núñez-Acuña, Camille Détrée, Cristian Gallardo-Escárate. Functional diets modulate lncRNAs-coding RNAs and gene interactions in the intestine of rainbow trout Oncorhynchus mykiss, European Aquaculture Society Conference 2017, CROACIA, Dubrovnik, 18 – 20 October.
  13. Gustavo Núñez-Acuña, Jorge Pino Marambio, Simon Wadsworth, Valentina Valenzuela-Muñoz, Sebastián Boltaña, Diego Valenzuela-Miranda, Ana Gonçalves, Cristian Gallardo. Discovering of key molecules involved in host recognition mechanisms in the salmon louse Caligus rogercresseyi, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 38.
  14. Ana Gonçalves, Cristian Gallardo, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Sebastián Boltaña, Diego Valenzuela-Miranda. Metabolic plasticity in Caligus rogercresseyi: one key for successful infestation, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 75.
  15. Rodolfo Farlora, Gustavo Núñez-Acuña, Flavio Valdebenito-Aguayo, Cristian Gallardo. Discovery and characterization of sex-related genes using high-throughput transcriptome sequencing from the salmon louse Caligus rogercresseyi, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 103.
  16. Sebastián Boltaña, Gustavo Núñez-Acuña, Andrea Aguilar, Cristian Gallardo, Diego Valenzuela-Miranda, Valentina Valenzuela-Muñoz, Ana Gonçalves. Switching responses in salmonids: skin regions exhibit quantitative and qualitative unlike response to caligidosis, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 10.
  17. Camille Détrée, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda, Valentina Valenzuela-Muñoz, Ana Gonçalves, Sebastián Boltaña, Cristian Gallardo-Escarate. Implication of lyzozymes of the atlantic salmon Salmo salar during the different stages of infection by Caligus rogercresseyi: A transcriptomic approach, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 109.
  18. Fredy Vera, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda, Ana Gonçalves, Camille Détrée, Sebastián Boltaña, Cristian Gallardo-Escárate. Prophenoloxidase system in the sea lice Caligus rogercresseyi, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 110.
  19. Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda, Ana Gonçales, Sebastián Boltaña, Cristian Gallardo. MicroRNA expression profiles in Atlantic salmon infected with Caligus rogercresseyi, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 111.
  20. Diego Valenzuela-Miranda, Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Ana Gonçalves, Sebastián Boltaña, Cristian Gallardo. Long non-coding RNAs: Unexplored players during drug metabolism in the sea lice Caligus rogercresseyi, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 32.
  21. Cristian Gallardo-Escarate, Sebastián Boltaña, Diego Valenzuela-Miranda, Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Rodolfo Farlora, Ana Gonçalves. The emerging role of non-coding RNAs in the host/sea lice interaction, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 35.
  22. Valentina Valenzuela-Muñoz, Sebastián Boltaña, Gustavo Núñez-Acuña, Diego Valenzuela-Miranda, Ana Gonçalves, Cristian Gallardo. Uncovering species-specific iron regulation patterns in Atlantic and Coho salmon during the infection with the sea louse Caligus rogercresseyi, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 48.
  23. Jorge Pino Marambio, Tirza Valenzuela, Gustavo Núñez-Acuña, Cristian Gallardo, Stanko Skugor, Helle Holm, Jorge Mancilla, Gordon Ritchie, Simon Wadsworth, In-feed additive with immunomodulatory effectn on Atantic salmon infested with sea lice Caligus rogercresseyi, 11th International Sea Lice Conference, IRLANDA, Westport, 2016, 71.
  24. Andrea Aguilar-Espinoza, Gustavo Núñez-Acuña, Cristian Gallardo-Escárate & Sebastian Boltaña. Salmonid species exhibit specific molecular responses against sea lice infections according to target zones in skin tissue. 3rd International Conference on Integrative Salmonid Biology. April 24 – 27, 2016, Puerto Varas, Chile.
  25. Cristian Gallardo-Escárate, Sebastian Boltaña, Diego Valenzuela-Miranda, Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz & Ana T. Gonçalves. In the Shadow: the emerging role of long non-coding RNAs in the immune response of Atlantic salmon. 4th International Symposium in Aquacultura GIA 2016. 20 – 22st April 2016, Athens, Greece.
  26. Gustavo Núñez-Acuña, Ana Teresa Gonçalves, Valentina Valenzuela-Muñoz, Jorge Pino Marambio, Simon Wadsworth & Cristian Gallardo Escárate. Transcriptome analysis from Atlantic salmon (Salmo salar): immunostimulants and their effects to salmon lice infection (Caligus rogercresseyi). 13th International Society of Developmental & Comparative Immunology (ISDCI). 28th June – 3rd July 2015, Murcia, Spain.
  27. Jorge Pino, Adnrés Quiroz, Christopher Hawes, Simon Wadsworth, Gustavo Núñez-Acuña and Cristian Gallardo-Escárate. Effects of in feed masking compounds on olfactory and immune response genes of Caligus rogercresseyi settlement in Atlantic salmon (Salmo salar). 10th International Sea Lice Conference, 31st August – 5th September 2014, Portland, Maine, USA.
  28. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Jacqueline Chávez-Mardones, Waleska Maldonado-Aguayo, Ana Teresa Gonçalves, Rodolfo Farlora, Diego Valenzuela-Miranda. Caligus rogercresseyi transcriptome: Novel insight for key biological processes during the lifecycle of the salmon louse. 10th International Sea Lice Conference, 31st August – 5th September 2014, Portland, Maine, USA.
  29. Gustavo Núñez-Acuña and Cristian Gallardo-Escárate. Insights into the olfactory system of the ectoparasite Caligus rogercresseyi: Molecular characterization and gene transcription analysis of novel olfactory transduction pathway. 10th International Sea Lice Conference, 31st August – 5th September 2014, Portland, Maine, USA.
  30. Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña, Andrea Peña & Cristian Gallardo-Escárate. Identification of candidate genes with response to delousing drugs by transcriptome mining in the salmon louse Caligus rogercresseyi. 10th International Sea Lice Conference, 31st August – 5th September 2014, Portland, Maine, USA.
  31. Valentina Valenzuela-Muñoz, Gustavo Núñez-Acuña & Cristian Gallardo-Escárate. RNA-seq analysis evidences a multiple gene response in Caligus rogercresseyi exposed to the organophosphate Azamethiphos (Bayer). 10th International Sea Lice Conference, 31st August – 5th September 2014, Portland, Maine, USA.
  32. Diego Valenzuela-Miranda, Gustavo Núñez-Acuña, Valentina Valenzuela-Muñoz, Cristian Gallardo-Escárate. miRNA Biogenesis pathway reveals transcriptional modulation in salmon louse (Caligus rogercresseyi) exposed to antiparasitic drugs. 10th International Sea Lice Conference, 31st August – 5th September 2014, Portland, Maine, USA.
  33. Cristian Gallardo-Escárate, Valentina Valenzuela-Muñoz, Gustavo Nuñez-Acuña, Ana Teresa Gonçalves*, Jacqueline Chavez-Mardones, Waleska Maldonado-Aguayo, Rodolfo Farlora. RNA-Seq analysis using de novo transcriptome assembly as a reference for the salmon louse Caligus rogercresseyi. World Aquaculture 2014, Adelaide.
  34. C. Gallardo-Escárate, V. Valenzuela-Muñoz, G. Nuñez-Acuña, J. Chávez-Mardones, W. Maldonado.Aguayo, A. T. Gonçalvez, R. Farlora. Caligus-seq: avances, desafíos y perspectivas sobre proyecto de secuenciación masiva de última generación en Caligus rogercresseyi. Foro Iberoamericano de los Recursos Marinos y la Acuicultura, 25-28 Noviembre, 2013. Valparaíso, Chile.
  35. Núñez-Acuña, G. & Gallardo-Escárate, C. “High-throughput transcriptome sequencing for SNPs discovery associated to immune-relevant genes in the mussel Mytilus chilensis”. 1st International Conference of Fish and Shellfish Immunology, June 25-28, 2013. Vigo, Spain.
  36. Gallardo-Escárate, C. & Núñez-Acuña, G. “RNA-seq analysis reveals a complex immune response against saxitoxin in the mussel Mytilus chilensis”. 1st International Conference of Fish and Shellfish Immunology, June 25-28, 2013. Vigo, Spain.
  37. Núñez-Acuña, G., Aballay, A.E., Astuya, A.P., Gallardo-Escárate, C. Transcriptome response of Mytilus galloprovincialis exposed in vivo to Saxitoxin (STX). XXXV Reunión Anual de la Sociedad de Bioquímica y Biología Molecular de Chile. 2-5 octubre 2012, Puerto Varas, Chile.
  38. Aguilar-Espinoza, A., Núñez-Acuña, G., Chávez-Mardones, J., Gallardo-Escárate, C. Ubiquitin-conjugating enzyme E2-like gene associated to pathogen response in Concholepas concholepas: SNP identification and transcription expression. XXXV Reunión Anual de la Sociedad de Bioquímica y Biología Molecular de Chile. 2-5 octubre 2012, Puerto Varas, Chile.
  39. Maldonado-Aguayo, W., Chávez-Mardones, J., Núñez-Acuña, G., Valenzuela-Muñoz., V., Gallardo-Escárate, C. Molecular characterization of two Serine Proteinase Inhibitor genes in the surf clam Mesodesma donacium exposed to Vibrio anguillarum. XXXV Reunión Anual de la Sociedad de Bioquímica y Biología Molecular de Chile. 2-5 octubre 2012, Puerto Varas, Chile.
  40. Chávez-Mardones, J., Núñez-Acuña, G., Maldonado-Aguayo, W., Valenzuela-Muñoz, V., Gallardo-Escárate, C. Ferritin characterization and identification of single nucleotide polymorphism (SNP) associated with innate immune response in Concholepas concholepas. XXXV Reunión Anual de la Sociedad de Bioquímica y Biología Molecular de Chile. 2-5 octubre 2012, Puerto Varas, Chile.
  41. Fabiola Lafarga-De la Cruz, Gustavo Núñez-Acuña, Cristian Gallardo-Escárate. Production of reciprocal hybrid abalones between the red abalones Haliotis rufescens and the Japanese abalone H. discus hannai. World Aquaculture Simposium. September 1-5. Prague, Czech Republic.
  42. Fabiola Lafarga-De la Cruz, Gabriel Amar-Basulto, Gustavo Núñez-Acuña, Valentina Valenzuela & Cristian Gallardo-Escárate. Haliotis rufescens x H. discus hannai: a new hybrid to improve the Chilean abalone aquaculture?. National Shellfish Research 2012. March 24-29. Seattle, USA.
  43. Núñez-Acuña, G., Aguilar-Espinoza, A., Haye, P. A., Cárdenas, L., Gallardo-Escárate, C. SNP markers discovery and validation in Concholepas concholepas. XLIV Reunión anual Sociedad de Genética de Chile. 6-9 de noviembre 2011, Puerto Varas, Chile.
  44. Alonso-Chivite, A., Amar-Basulto, G., Valenzuela-Muñoz, V., Núñez-Acuña, G., Aguilar-Espinoza, A., Gallardo-Escárate, C. Molecular identidfication of abalone hybrids using VER gene: RFLP and FISH analysis. XLIV Reunión anual Sociedad de Genética de Chile. 6-9 de noviembre 2011, Puerto Varas, Chile.
  45. Núñez-Acuña, G., Aguilar-Espinoza, A., Haye, P. A., Cárdenas, L., Gallardo-Escárate, C. Novel genomic resources for aquaculture and restocking of Concholepas concholepas (neogastropoda: muricidae). Genomics in Aquaculture International Symposium (GIA 2011). 14-17 September 2011, Heraklion, Crete, Greece.
  46. Aguilar-Espinoza, A., Núñez-Acuña, G., Valenzuela-Muñoz, V., Gallardo-Escárate, C. Transcriptome characterization in Mesodesma donacium (Bivalvia) and discovery of new molecular markers. Genomics in Aquaculture International Symposium (GIA 2011). 14-17 September 2011, Heraklion, Crete, Greece.
  47. Núñez-Acuña, G., Aguilar-Espinoza, A., Haye, P. A., Cárdenas, L., Gallardo-Escárate, C. High-throughput transcriptome sequencing from concholepas concholepas: genomic resources for restocking and aquaculture. World Aquaculture 2011, 07-10 june, Natal, Brazil.
  48. Núñez-Acuña, G., Valenzuela-Muñoz, V., P. Haye-Molina & Gallardo-Escárate, C. Analysis of gene expression patterns in Mytilus chilensis in aquaculture zones from Southern Chile. III International Aquaculture Congress, 22-26 november 2010, Viña del Mar, Chile.
  49. Salinas-Clarot, K., Núñez-Acuña, G. & Gallardo-Escárate, C. Molecular characterization and gene expression analysis of ferritin in red abalone (Haliotis rufescens) in response to thermal stress. III International Aquaculture Congress, 22-26 november 2010, Viña del Mar, Chile.
  50. Nuñez-Acuña, G., Castillo-Lara, A. & C. Gallardo-Escárate. “HSP70 gene expression analysis in hybrids between Haliotis rufescens and Haliotis discus hannai exposed to thermal stress”. Congreso COLACMAR, 26-30 Octubre 2009, Habana-Cuba.
  51. Castillo-Lara, A., Valenzuela-Muñoz, V., Nuñez-Acuña, G. & Gallardo-Escárate, C. “Gene expression analysis of catalase and super-oxide dismutase in Haliotis rufescens exposed to oxidative stress”. Congreso COLACMAR, 26-30 Octubre 2009. Habana-Cuba.
  52. Núñez-Acuña, G., Castillo-Lara, A., Gallardo-Escárate, C. “HSP70 gene expression analysis in hybrids between Haliotis rufescens and Haliotis discus hannai exposed to thermal stress”. XLII Reunión Anual de la Sociedad de Genética de Chile. 21-23 Octubre 2009, Hotel El Dorado, Concepción-Chile.
  53. Castillo-Lara, A., Valenzuela-Muñoz,V., Núñez-Acuña, G., Gallardo-Escárate, C. “Gene expression analysis of catalase and super-oxide dismutase in Haliotis rufescens exposed to oxidative stress”. XLII Reunión Anual de la Sociedad de Genética de Chile. 21-23 Octubre 2009, Hotel El Dorado, Concepción-Chile.
Dra. Valentina Valenzuela

Dra. Valentina Valenzuela

valevalenzuela@gmail.com

Investigadora Postdoctoral  RP1

Publicaciones
Congresos
René Manríquez Vásquez

René Manríquez Vásquez

rene.manriquez@uach.cl

Investigador Postdoctoral RP3

Doctor en Ciencias de la Acuicultura, Universidad Austral de Chile.

Licenciado en Bioquímica, Universidad Austral de Chile.

Bioquímico, Universidad Austral de Chile.

Publicaciones

Publicaciones

  1. Cárcamo JG, Manríquez RA, Vera T, Avendaño-Herrera R. American Association of Immunologists Congress. May 2015. New Orleans. (2015). Epigenetic modulation of immune response in salmonid cells infected with the infectious pancreatic necrosis virus (IPNV)., USA. J Immunol 2015. 194:75.12. (Supplement 1).
  2. Juan Guillermo Cárcamo, René Manríquez, Cristián Torres, Alejandra Mancilla, Melina Villalba, Alejandro Yañez. 2013 Revista versión diferente Salmon-acuícola. Año 10 N°18 pág. 26-29. Vacuna oral basada en partículas virales vacías (VLPs, Virus Like Particles) contra el virus de la Necrosis Pancreática Infecciosa (IPNv).
  3. Álvarez, C. Rojas, R. Manríquez, G. Kausel, J. Figueroa, R. Enríquez, M. Monrás, A. Romero. Comparative Biochemistry and Phisiology , Volume 154ª, Number1/Suppl. (2009) s25,8 :Cloning and characterization of cortisol receptor from Salmo salar and analyses of its role in fish leukocytes.
  4. Alex Romero, Claudio Álvarez, René Manríquez, Mauricio López, Claudio Rojas, Jaime Figueroa, Mónica Monrás y Ricardo Enríquez. 2009 Revista versión diferente Salmon-acuícola. Año 6 N°11 pág. 31-33. Estrés en peces y su efecto en el sistema inmune.

Publicaciones ISI

  1. René A Manríquez, Tamara Veraa, Melina V Villalba, Alejandra Mancillaa, Vikram N Vakharia, Alejandro J Yañeza, Juan G Cárcamo, A Molecular characterization of infectious pancreatic necrosis virus strains isolated from the three types of salmonids farmed in Chile. Virology Journal (2017) 14:17 DOI 10.1186/s12985-017-0684-x
  2. Alex Romero, René Manríquez, Mauricio López, Gudrun Kausel, Hugo Silva, Mónica Monrás, Jorge Vásquez, Alejandro Yáñez, Ricardo Enríquez, Roxana González-Stegmaier and Jaime Figueroa. New inmunomodulatory role of neuropeptide Y (NPY) in leucocytes of Salmo salar. Dev Comp Immunol. 2017 Nov;76:303-309. doi: 10.1016/j.dci.2017.06.018. Epub 2017 Jul 1.
  3. Alex Romero, René Manríquez, Claudio Álvarez, Cristina Gajardo, Jorge Vásquez, Gudrun Kausel, Mónica Monrás, Víctor H. Olavarría, Alejandro Yáñez, Ricardo Enríquez, Jaime Figueroa. Prolactin-releasing peptide is a potent mediator of the innate immune response in leukocytes from Salmo salar. Veterinary Immunology and Immunopathology. Manuscript Number: VETIMM-D-12-16R1 Veterinary Immunology and Immunopathology Volume 147, Issues 3–4, 30 June 2012, Pages 170–179 http://dx.doi.org/10.1016/j.vetimm.2012.04.014
  4. Cárcamo JG, Aguilar MN, Barrientos CA, Carreño CF, Quezada CA, Bustos C, Manríquez RA, Avendaño-Herrera R, Yañez Aj. (2011) Effect of emamectin benzoate on transcriptional expression of cytochromes P450 and the multidrug transporters (Pgp and MRP1) in rainbow trout (Oncorhynchus mykiss) and the sea lice Caligus rogercresseyi. Aquaculture, online publication complete: 4-OCT-2011. DOI information: 10.1016/j.aquaculture.2011.09.012. Volume 321, Issues 3–4, 1 December 2011, Pages 207–215 http://dx.doi.org/10.1016/j.aquaculture.2011.09.012
Proyectos
  1. FONDECYT 3170881. PostDoctorado. Investigador responsable. Epigenoma de la infección con el virus de la necrosis pancreática infecciosa (IPNV) en Salmón del Atlántico (Salmo salar): Identificación de marcadores epigenéticos para el desarrollo de blancos terapéuticos.
  2. FONDEF VIU16P0165. Investigador responsable. Sexto Concurso de Valorización de la Investigación en la Universidad. Moduladores epigenéticos para la acuicultura.
Congresos
  1. Vera, Tamara, Manríquez René, Cárcamo Juan Guillermo, Cytopathogenicity levels in different Chilean isolates of infectious pancreatic necrosis virus (IPNV). XXXVI Congreso Chileno de Microbiología, La Serena, Chile.
  2. Jara, Ronald, Manríquez, René, Figueroa, Jaime, Molina, Alfredo, Cárcamo, Juan Guillermo, Avendaño, Ruben, Enríquez, Ricardo, Monrás, Monica, Gonzalez-Stegmaier, Roxana, Romero, Alex. IPNv con distinto grado de virulencia modulan de forma diferencial la expresión de genes de respuesta antiviral y desarrollo muscular en truchas arcoiris (Oncorhynchus mykiss) XXXVI Congreso Chileno de Microbiología, La Serena-Chile.
  3. Cárcamo JG, Vera T, Manríquez R. Kinetic Characterization of Levels of Cytopathogenicity of Different Chilean Isolates of Pancreatic Necrosis Virus (IPNV). 23rd Congress of the International Union for Biochemistry and Molecular Biology (IUBMB-2015). 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology. Foz do Iguazu, Brasil. August 24-28, 2015.
  4. Manríquez R. Avendaño-Herrera, T. Vera, J.G.Cárcamo. The Cytokines Expression is Modulated by Epigenetic Mechanisms in Response to Infection with IPNV. 6th Congress of European Microbiologists (FEMS 2015 Congress) Maastrich-Holanda.
  5. Manríquez, O. Monroig , A. Farías, I. Uriarte, A. Romero, R. AvendañoHerrera, J.G.Cárcamo. Identificación de Enzimas involucradas en la Biosíntesis de Ácidos Grasos Esenciales en Enteroctopus Megalocyathus. VI Foro Iberoamericano de los Recursos Marinos y la Acuicultura (FIRMA). 25-28 Noviembre 2013 Valparaiso-Chile.
  6. J.P. Cumillaf, R. Manriquez, C. Barrientos, H. Silva, A. Romero, P. Gebauer, K. Paschke. Effects of hypoxia on the physiological and immune responses, and on the haemocyanin mRNA expression levels in reared juveniles of the southern king crab Lithodes Santolla (Molina, 1782) (Decapoda: Lithodidae). Aquaculture conference: To the Next 40 Years of Sustainable Global Aquaculture 3rd -7th November 2013 Palacio de Congresos de Canarias, Las Palmas, Gran Canaria
  7. Cumillaf JP, Manriquez R, Barrientos C, Silva H, Romero A, Gebauer P y Paschke K. Efecto de la concentración de oxigeno sobre respuestas fisiológicas e inmune y sobre la expresión transcripcional de hemocianina, en juveniles de centolla Lithodes santolla (molina, 1782) (decápoda: lithodidae) 16-18 de Enero 2013, IV Congreso Nacional de Acuicultura, Puerto Montt-Chile.
  8. Juan Guillermo Cárcamo, René Manríquez, Melina Villalba, Alejandra Mancilla, Cristián Torres, Alejandro Yañez, Alex Romero. Vacuna VLPS (Virus Like Particles) para aislados chilenos de IPNV. 23-26 DE NOVIEMBRE, 2012 XXXIV Congreso Chileno de Microbiología.
  9. Juan G. Cárcamo, René A. Manríquez, Cristian I. Torres, Alejandra Mancilla, Vikram Vakharia and Alejandro Yañez. Production of VLPs of infectious pancreatic necrosis virus (IPNV) using the baculovirus-insect cell expression system. November 27 – December 1, 2012 11th International Symposium on Double-Stranded RNA Viruses, San Juan-Puerto Rico.
  10. Romero A., Manríquez R., López M., Kausel G., Silva H., Monrás M., Figueroa J., Enríquez R. Identificación del neuropéptido Y (NPY) en el sistema inmune de Salmo salar: análisis de su expresión y participación en la actividad de leucocitos 18-20 de noviembre 2012 17° Congreso de medicina veterinaria, Valdivia-Chile
  11. Manríquez R.A.*, Moreno I., Araya E, Olavarria V., Yañez A. Cárcamo J.G. Determinación del tamaño de partículas virales utilizando el equipo Nanosizer 18-20 de noviembre 2012 17° Congreso de medicina veterinaria, Valdivia-Chile
  12. Rene A. Manríquez, Melina V. Villalba, Freddy A. Calderón, Juan G. Cárcamo. Laboratorio de Bioquímica Farmacológica, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile.1-5 November, 2011 Chilean Society for cell biology XXV annual meeting, Puerto varas, Chile. Production of VLPs of infectious pancreatic necrosis virus (IPNV) by using baculovirus system.
  13. Carreño C, Barrientos CA, Aguilar M, Castilla S, Manríquez R, Villalba M, Cárcamo JG, Yáñez A. Universidad Austral de Chile.27- 30 sept, 2011 XXXIV Reunión Anual Sociedad De Bioquímica y Biología Molecular de Chile, Hotel Dreams Pedro de Valdivia: Changes in protein expresión levels of metabolism and drug resistance proteins after treatment with flumequine and florfenicol antibiotics in Chinook salmon embryo cells (CHSE-214) cultured in vitro.
  14. Calderón F, Barrientos CA, Manríquez R, Quezada C, Yánez A, Cárcamo JG. Universidad Austral de Chile.27- 30 sept, 2011XXXIV Reunión Anual Sociedad De Bioquímica y Biología Molecular de Chile, Hotel Dreams Pedro de Valdivia:Effect of Temodal on the expression level of Major Vault Protein (MVP) in a Glioblastoma Multiforme cell line.
  15. Barrientos CA, Aguilar MN, Carreño CF, Villalba MV, Manríquez R, Castilla SM, Calderón FA, Yáñez AJ, Cárcamo JG. Universidad Austral de Chile.27- 30 sept, 2011XXXIV Reunión Anual Sociedad De Bioquímica y Biología Molecular de Chile, Hotel Dreams Pedro de Valdivia: Effect of Flumequin and Florfenicol on Expression in Metabolic Proteins and Drug Resistance Proteins in Head Kidney Cells of Atlantic salmon (SHK-1) cultured in vitro
  16. Carreño CF, Barrientos CA, Villalba MV, Manríquez R, Castilla SM, Aguilar MN, Cárcamo JG. Universidad Austral de Chile.11-13 mayo 2011:II Congreso Latinoamericano de Genética Humana, VI Congreso Nacional de Biología y II Congreso Costarricense de Biotecnología, San José de Costa Rica: Alteración de los niveles de expresión de enzimas del complejo citocromo P450 y proteínas MDR en células embrionarias de salmón Chinook (CHSE-214) cultivadas in vitro post tratamientos con antibióticos.
  17. Villalba M, Manríquez R, Castilla S, Carreño CF, Barrientos CA, Cárcamo JG. Universidad Austral de Chile.11-13 mayo 2011:II Congreso Latinoamericano de Genética Humana, VI Congreso Nacional de Biología y II Congreso Costarricense de Biotecnología, San José de Costa Rica: Caracterización molecular de aislados chilenos del virus de la necrosis pancreática infecciosa.
  18. Barrientos CA, Carreño CF, Villalba MV, Manríquez R, Castilla SM, Aguilar MN, Cárcamo JG. Universidad Austral de Chile.11-13 mayo 2011:II Congreso Latinoamericano de Genética Humana, VI Congreso Nacional de Biología y II Congreso Costarricense de Biotecnología, San José de Costa Rica: Efecto de antibióticos sobre la expresión de proteínas de metabolización y de resistencia a drogas en células de riñón anterior de salmón del Atlántico (SHK-1) cultivadas in vitro.
  19. Manríquez, R.; López, M.; Kausel, G.; Silva, H.; Monrás, M.; Enríquez, R; Romero, A. 4-5 Mayo 2011: 1º Reunión Científica de la Facultad de Ciencias Veterinarias, Valdivia: Identificación del Receptor de NPY en el Sistema Inmune de Salmo salar: Análisis de su Expresión y Participación en la Actividad de Leucocitos.
  20. Romero, A., Manríquez, R., Vásquez, J., Villatoro, J.M., Rojas, C., Alvarez, C., Muller, M., Kausel, G., Figueroa, J., Monrás, M. and Enríquez, R. 5-9 de Julio de 2010: 9th International Congress on the Biology of Fish, , Barcelona-España: New biological activity of prolactin releasing peptide CRFA in immune cells of Salmo salar.
  21. López, M.; Manríquez, R.; Kausel, G.; Monrás, M.; Figueroa, J; Romero, A. Identificación y caracterización del neuropéptido Y de Salmo salar expresado en leucocitos. Pag.51. 16-20 de noviembre de 2009.: LII Reunión Anual de Sociedad de Biología de Chile. Pucón, Chile.
  22. Manríquez, R.; López, M.; Kausel, G.; Silva, H.; Monrás, M.; Enríquez, R; Romero, A. Identificación del receptor de NPY en el sistema inmune de Salmo salar: Análisis de su expresión y participación en la actividad de leucocitos. Pag.50.16-20 de noviembre de 2009.: LII Reunión Anual de Sociedad de Biología de Chile. Pucón, Chile.
  23. C. Álvarez, C. Rojas, R. Manríquez, G. Kausel, J. Figueroa, R. Enríquez, M. Monrás, A. Romero. 6-9 septiembre 2009: 26 congress of the European society of Comparative Biochemistry and Physiology. Insbruck, Austria: Cloning and characterization of cortisol receptor form Salmo salar and analyses of its role in fish leukocytes.Pag.S25.
Rodrigo Montes

Rodrigo Montes

rmontes@oceanografia.udec.cl

Investigador Postdoctoral  RP4

Publicaciones
Proyectos
Congresos